Simple explanation of the clinical trial science
The study in the document tested whether a hair-care formula containing biotin, and caffeine can improve hair density and growth conditions.
(Bioboosting feed Biotin.... ⤷Clinical Results link)
To do this, the researchers performed two main types of scientific tests:
1. Ex vivo penetration study – to see if ingredients reach the hair roots
2.In vivo human study – to see if people’s hair improves
Think of it as:
Step 1: Does the ingredient reach the target?
Step 2: Does it help hair grow?
1. Ex vivo penetration study (how ingredients reach the hair root)
What “ex vivo” means
“Ex vivo” means the test is done on human skin samples outside the body (in a lab).
Scientists use this to see how deeply ingredients penetrate the scalp.
The problem they are testing
Hair products often sit on the surface of the scalp.
But to work properly, ingredients must reach the hair follicle, where hair grows.
The product uses liposomes (tiny fat bubbles) to carry ingredients deeper.
Bioboosting feed Biotin …
How they tested penetration
Researchers used special glowing markers:
|
Marker |
What it represents |
|
CF (Carboxyfluorescein) |
water-loving ingredient |
|
DiL |
oil-loving ingredient |
These markers glow under laser microscopes.
They mixed them with:
- normal solution
- the liposome delivery system
Then they applied them to human skin samples.
After 8 hours and 16 hours, they examined the skin.
The imaging technology
They used:
Confocal laser scanning microscopy
Think of it like:
- a super powerful microscope
- that scans thin layers of skin
- and shows exactly where the glowing molecules went
It allows scientists to see if ingredients reached the:
- scalp
- hair follicle
- hair root
Result (in simple terms)
The liposome system delivered ingredients deeper into the hair root area.
Bioboosting feed Biotin …
So, the delivery system works better than normal solutions.
2. Human study (in vivo trial)
After confirming the ingredients reach the hair roots, researchers tested real people.
Participants
12 volunteers:
- 6 men
- 6 women
- age 31–60
- all had diffuse hair loss.
Bioboosting feed Biotin …
What they did
Participants used the hair tonic:
- once per day
- for 24 weeks (about 6 months)
Researchers measured hair changes at:
- 6 weeks
- 12 weeks
- 18 weeks
- 24 weeks
How they measured hair scientifically (TrichoScan)
The key technology used is:
TrichoScan
This is one of the most common clinical hair measurement methods.
How TrichoScan works
- A small area of scalp is shaved (about 1 cm²).
- After 2–3 days, hair begins to grow.
- A high-resolution microscope camera takes pictures.
- Computer software automatically analyzes:
number of hairs
- growth speed
- growth phase
What TrichoScan measures
1. Hair density
How many hairs exist in a specific area.
Example:
150 hairs per cm²
If this number increases, it means more hair growth.
2. Hair growth phases
Hair grows in cycles:
|
Phase |
Meaning |
|
Anagen |
active growth |
|
Catagen |
transition |
|
Telogen |
resting / shedding |
Healthy scalp:
~80–90% growing (anagen)
~10–20% resting (telogen)
Hair loss conditions often show:
too many hairs in telogen
The study measured the anagen/telogen ratio.
Results explained simply
After 24 weeks:
Hair growth cycle improved
Ratio moved toward healthy levels:
Before: 69% growing hair
After: 80% growing hair
Meaning more follicles were actively producing hair.
Bioboosting feed Biotin …
Hair density increased
Men:
+9.5% more hairs
Women:
+16% more hairs
This suggests more visible hair thickness.
Bioboosting feed Biotin …
Self-assessment (participant feedback)
Participants also rated:
- hair volume
- shine
- scalp feeling
- product texture
Scores were generally positive (close to “very good”).
Cosmetic study
The document itself states it is not a drug trial.
Bioboosting feed Biotin …
So, it is more like a cosmetic efficacy study.
Very simple summary
The study followed a typical cosmetic science approach:
Step 1 — Lab test
Use glowing markers and microscopes to confirm the product can reach hair roots.
Step 2 — Human study
12 people used the product for 6 months.
Scientists measured hair with TrichoScan imaging software.
Results
More hairs in growth phase
Hair density increased
Participants reported improvement
